The Bartholin glands are small almond shaped organs located on both sides of the vaginal opening that produce essential mucus for lubrication. If a duct becomes blocked due to infection or irritation, fluid Your Vagina: Everything You Need to Know What Is Vaginal Lubrication Made Of and Where Does It Come From? The Bartholin's gland is located at the entrance to the vagina, and it secretes mucus, providing vulval and vaginal lubrication. A Bartholin's cyst occurs when the gland becomes blocked, and this can Vaginal Discharge Types, Causes, Symptoms, Treatment, Prevention The Cervicovaginal Mucus Barrier